	Username:		Password:

0 (item/s)

Quick Search :
Advanced

TSW Editorial Network

Authors


TheScientificWorldJOURNAL (ISSN 1537-744X)

Article Details

Title:

Transcriptional Profiling of Caudal Fin Regeneration in Zebrafish

Authors:

Schebesta, Michael ; Lien, Ching-Ling ; Engel, Felix B.; Keating, Mark T.

Journal:

TheScientificWorldJOURNAL

Year:

2006

Volume:

Supplement no.:

Page Range:

38-54

Article Type:

Research Article

Handling Editor:

Marie-Andree Akimenko

Domains:

Neuroscience , Cell Fate & Determination , Cell Biology , Experimental Medicine , Motor Processes , Cell Signaling , Development & Embryology , Genes & Genomics , Intercellular Communication , Biochemistry & Molecular Biology

DOI:

10.1100/tsw.2006.326

Article clouds not enabled. Press the image to enable for this session.

Synopsis:

Regenerative medicine, rebuilding organs and tissues, is an important topic in biomedical research. Nevertheless, little is known about the fundamental principles underlying regeneration. To provide new insight, we used the zebrafish fin regeneration model and performed a microarray screen, analyzing expression profiles of 15,000 transcripts during regeneration. We present a comprehensive database of differentially regulated transcripts. In addition to expression data, our database contains functional descriptions and background information about those transcripts.

Keywords:

regeneration, fin, zebrafish, microarray, bmp, bambi, dlx, her

[Related TSW Articles] [Export to EndNote] [Open Choice]

Abstract

Regeneration of severed limbs in adult animals is restricted to urodele amphibians. Mammals, including humans, have very limited regenerative capabilities and even with proper treatment, only the tips of our digits can grow back. Teleost fish can regenerate amputated fins, the evolutionary ancestors of limbs. To elucidate the principles of limb-fin regeneration, we performed an Affymetrix microarray screen on regenerating caudal fins 12, 24, 48, and 72 h post amputation. Approximately 15,000 zebrafish transcripts were analyzed, identifying 829 transcripts as differentially expressed during regeneration. Of those, 563 were up-regulated and 266 were down-regulated. We constructed a comprehensive database containing expression data, functional assignment, and background information from the literature for each differentially expressed transcript. In order to validate our findings, we employed three approaches: (1) microarray expression analysis of genes previously implicated in fin regeneration, (2) RT-PCR analysis of genes newly identified as differentially expressed during regeneration, and (3) in situ hybridization of the up-regulated genes bambi, dlx5A, and her6. Moreover, we show that Smad 1/5/8 proteins, effector molecules of Bmp signaling, are phosphorylated during fin regeneration. Taken together, we provide a comprehensive database of fin regeneration that will serve as an important tool for understanding the molecular mechanisms of regeneration.

TheScientificWorldJOURNAL (ISSN 1537-744X)

Article Details